AG Hamprecht

Group Leader: Univ. Prof. Dr. med. Axel Hamprecht

Antibiotika-Resistenz bei Gram-negativen Pathogenen
Phone: +49 221 478-32009 (Sekretariat)
E-Mail: [Email protection active, please enable JavaScript.]

Co-operation partners at IMMIH
Dr. med. Janko Sattler, [Email protection active, please enable JavaScript.]
Tsvetan Tsvetkov, [Email protection active, please enable JavaScript.]

Research Topic:
Resistance mechanisms in gram-negative bacteria

Detection and resistance of medically important fungi

Resistance mechanisms in gram-negative bacteria
Resistance to antibiotics has dramatically increased in recent years, especially among gram-negative bacteria (e.g., Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa). These bacteria cause life threatening infections (e.g. sepsis, pneumonia) and can be transmitted from patient to patient.

In the last years, we witness an increasing resistance to commonly used beta-lactam antibiotics, especially to extended spectrum cephalosporins. Resistance is commonly caused by extended spectrum beta lactamases that can hydrolyze many beta lactams.

Another threat which is still rare in Germany is the resistance to carbapenems (e.g., ertapenem, meropenem, imipenem), which is caused by carbapenemases such as NDM (New Delhi metallo-beta-lactamase), KPC, VIM, OXA-48 and others.

The main interest of our group is to identify and characterize resistant Gram-negative bacteria and to understand why some species and clones can disseminate successfully.

Furthermore, we work on the rapid detection of carbapenemase-producing Enterobacteriaceae directly from clinical specimen.

Azole-resistant Aspergillus fumigatus (TR46/Y121F/T289A mutation)

Detection and resistance of medically important fungi
With the growing number of immune-compromised patients, we see an increasing number of infections by opportunistic pathogens, especially fungi. Invasive fungal infections such as aspergillosis show a very high mortality and are particularly difficult to treat. This problem has been aggravated by an increasing resistance to triazole antifungals, the mainstay of aspergillosis therapy. This phenomenon has only been observed in the last years and is likely caused the use of azole fungicides in agriculture.

Our current research focuses on azole resistance in Aspergillus spp. and the detection and resistance of “rare” fungi, such as Fusarium spp.

Brief description in German
Unsere Arbeitsgruppe beschäftigt sich mit Resistenzen und Resistenzmechanismen von gram-negativen Bakterien und medizinisch wichtigen Pilzen.

Resistenzen gegenüber Antibiotika und Antimykotika haben in den letzten Jahren dramatisch zugenommen. Dies ist u.a. bedingt durch den zu häufigen Einsatz von Antibiotika (Klinik, ambulant, Tierhaltung) und von Antimykotika (Landwirtschaft).

Unsere Arbeitsgruppe hat sich zum Ziel gesetzt, resistente Stämme nachzuweisen und zu charakterisieren sowie die zugrundeliegenden Resistenzmechanismen aufzuklären. Unser Hauptinteresse liegt hierbei bei Antibiotikaresistenzen gegenüber Betalaktamen (z.B. durch ESBLs oder Carbapenemasen wie NDM, KPC, OXA-48 u.a.) und Antimykotikaresistenzen bei Aspergillus spp. gegenüber Triazolen.

Bachelor-, Master-, und Diplomarbeiten

Selected publications

Göttig S, Walker SV, Saleh A, Koroska F, Sommer J, Stelzer Y, Steinmann J, Hamprecht A.
Comparison of nine different selective agars for the detection of carbapenemase-producing Enterobacterales (CPE).
Eur J Clin Microbiol Infect Dis.
2020 Jan 4. doi: 10.1007/s10096-019-03786-7. [Epub ahead of print] PubMed PMID: 31902017.
 
Hamprecht A, Sommer J, Willmann M, Brender C, Stelzer Y, Krause FF, Tsvetkov T, Wild F, Riedel-Christ S, Kutschenreuter J, Imirzalioglu C, Gonzaga A, Nübel U,
Göttig S.
Pathogenicity of Clinical OXA-48 Isolates and Impact of the OXA-48 IncL Plasmid on Virulence and Bacterial Fitness. Front Microbiol. 2019 Nov 1;10:2509.
doi: 10.3389/fmicb.2019.02509. eCollection 2019. PubMed PMID: 31736929; PubMed
Central PMCID: PMC6838017.
 
Jakob CEM, Stecher M, Claßen AY, Hamprecht A, Cornely OA, Vehreschild JJ.
Association Between Prescribed Opioids and Infections in Patients With
Neutropenia and Cancer. JAMA Intern Med. 2019 Nov 4. doi:
10.1001/jamainternmed.2019.4714. [Epub ahead of print] PubMed PMID: 31682679;
PubMed Central PMCID: PMC6830429.
 
Willmann M, Vehreschild MJGT, Biehl LM, Vogel W, Dörfel D, Hamprecht A, Seifert H, Autenrieth IB, Peter S.
Distinct impact of antibiotics on the gut microbiome and resistome: a longitudinal multicenter cohort study. BMC Biol. 2019
Sep 18;17(1):76. doi: 10.1186/s12915-019-0692-y. PubMed PMID: 31533707; PubMed
Central PMCID: PMC6749691.
 
Meier M, Hamprecht A.
Systematic Comparison of Four Methods for Detection of
Carbapenemase-Producing Enterobacterales Directly from Blood Cultures. J Clin
Microbiol. 2019 Oct 23;57(11). pii: e00709-19. doi: 10.1128/JCM.00709-19. Print
2019 Nov. PubMed PMID: 31413083; PubMed Central PMCID: PMC6813004.
 
Lucena Baeza L, Pfennigwerth N, Hamprecht A.
Rapid and Easy Detection of Carbapenemases in Enterobacterales in the Routine Laboratory Using the New
GenePOC Carba/Revogene Carba C Assay. J Clin Microbiol. 2019 Aug 26;57(9). pii:
e00597-19. doi: 10.1128/JCM.00597-19. Print 2019 Sep. PubMed PMID: 31315949;
PubMed Central PMCID: PMC6711903.
 
Fuchs F, Hamprecht A.
Susceptibility of carbapenemase-producing Enterobacterales (CPE) to nitroxoline. J Antimicrob Chemother. 2019 Oct
1;74(10):2934-2937. doi: 10.1093/jac/dkz275. PubMed PMID: 31292653.
 
Hamprecht A, Barber AE, Mellinghoff SC, Thelen P, Walther G, Yu Y, Neurgaonkar P, Dandekar T, Cornely OA, Martin R, Kurzai O; German Candida auris Study Group.
Candida auris in Germany and Previous Exposure to Foreign Healthcare. Emerg
Infect Dis. 2019 Sep;25(9):1763-1765. doi: 10.3201/eid2509.190262. Epub 2019 Sep
17. PubMed PMID: 31223105; PubMed Central PMCID: PMC6711216.
 
Fuchs F, Hamprecht A.
Results from a Prospective In Vitro Study on the Mecillinam (Amdinocillin) Susceptibility of Enterobacterales.
Antimicrob Agents Chemother. 2019 Mar 27;63(4). pii: e02402-18. doi: 10.1128/AAC.02402-18. Print
2019 Apr. PubMed PMID: 30917983; PubMed Central PMCID: PMC6437539.
 
Baeza LL, Pfennigwerth N, Greissl C, Göttig S, Saleh A, Stelzer Y, Gatermann SG, Hamprecht A.
Comparison of five methods for detection of carbapenemases in Enterobacterales with proposal of a new algorithm. Clin Microbiol Infect. 2019
Oct;25(10):1286.e9-1286.e15. doi: 10.1016/j.cmi.2019.03.003. Epub 2019 Mar 18.
PubMed PMID: 30898725.
 
Biehl LM, Higgins P, Wille T, Peter K, Hamprecht A, Peter S, Dörfel D, Vogel W, Häfner H, Lemmen S, Panse J, Rohde H, Klupp EM, Schafhausen P, Imirzalioglu C,
Falgenhauer L, Salmanton-García J, Stecher M, Vehreschild JJ, Seifert H, Vehreschild MJGT.
Impact of single-room contact precautions on hospital-acquisition and transmission of multidrug-resistant Escherichia coli: a
prospective multicentre cohort study in haematological and oncological wards.
Clin Microbiol Infect. 2019 Aug;25(8):1013-1020. doi: 10.1016/j.cmi.2018.12.029.
Epub 2019 Jan 12. PubMed PMID: 30641228.
 
Greissl C, Saleh A, Hamprecht A.
Rapid detection of OXA-48-like, KPC, NDM, and VIM carbapenemases in Enterobacterales by a new multiplex
immunochromatographic test. Eur J Clin Microbiol Infect Dis. 2019
Feb;38(2):331-335. doi: 10.1007/s10096-018-3432-2. Epub 2018 Nov 17. PubMed PMID:
30448931.
 
Hamprecht A, Vehreschild JJ, Seifert H, Saleh A.
Rapid detection of NDM, KPC and OXA-48 carbapenemases directly from positive blood cultures using a new
multiplex immunochromatographic assay. PLoS One. 2018 Sep 14;13(9):e0204157. doi:
10.1371/journal.pone.0204157. eCollection 2018. PubMed PMID: 30216371; PubMed
Central PMCID: PMC6138386.
Pietsch M, Irrgang A, Roschanski N, Brenner Michael G, Hamprecht A, Rieber H, Käsbohrer A, Schwarz S, Rösler U, Kreienbrock L, Pfeifer Y, Fuchs S, Werner G; RESET Study Group.
Whole genome analyses of CMY-2-producing Escherichia coli isolates from humans, animals and food in Germany.
BMC Genomics. 2018 Aug 9;19(1):601. doi: 10.1186/s12864-018-4976-3. PubMed PMID: 30092762
 
Russo A, Falcone M, Gutiérrez-Gutiérrez B, Calbo E, Almirante B, Viale PL, Oliver A, Ruiz-Garbajosa P, Gasch O, Gozalo M, Pitout J, Akova M, Peña C, Cisneros JM, Hernández-Torres A, Farcomeni A, Prim N, Origüen J, Bou G, Tacconelli E, Tumbarello M, Hamprecht A, Karaiskos I, de la Calle C, Pérez F, Schwaber MJ, Bermejo J, Lowman W, Hsueh PR, Mora-Rillo M, Rodriguez-Gomez J, Souli M, Bonomo RA, Paterson DL, Carmeli Y, Pascual A, Rodríguez-Baño J, Venditti M; REIPI/ESGBIS/INCREMENT investigators.
Predictors of outcome in patients with severe sepsis or septic shock due to extended-spectrum β-lactamase-producing Enterobacteriaceae.
Int J Antimicrob Agents. 2018 Jun 30. pii: S0924-8579(18)30186-9. doi: 10.1016/j.ijantimicag.2018.06.018. [Epub ahead of print] PubMed PMID: 29969692.
 
Seufert R, Sedlacek L, Kahl B, Hogardt M, Hamprecht A, Haase G, Gunzer F, Haas A, Grauling-Halama S, MacKenzie CR, Essig A, Stehling F, Sutharsan S, Dittmer S, Killengray D, Schmidt D, Eskandarian N, Steinmann E, Buer J, Hagen F, Meis JF,Rath PM, Steinmann J.
Prevalence and characterization of azole-resistant Aspergillus fumigatus in patients with cystic fibrosis: a prospective multicenter study in Germany.
J Antimicrob Chemother. 2018 Aug 1;73(8):2047-2053. doi: 10.1093/jac/dky147. PubMed PMID: 29684150.
 
Thelen P, Hornberg K, Hamprecht A.
Efficient processing of MRSA screening specimens by a modified inoculation protocol.
Eur J Clin Microbiol Infect Dis. 2018 Jul 6. doi: 10.1007/s10096-018-3319-2. PubMed PMID: 29980897.
 
Hamprecht AG, Göttig S.
Häufigkeit und Vorkommen multiresistenter gramnegativer Erreger: 3MRGN/4MRGN.
Dtsch Med Wochenschr. 2018 May;143(9):625-633. doi: 10.1055/s-0043-113597. PubMed PMID: 29684929.
 
Saleh A, Göttig S, Hamprecht AG.
Multiplex Immunochromatographic Detection of OXA-48, KPC, and NDM Carbapenemases: Impact of Inoculum, Antibiotics, and Agar.
J Clin Microbiol. 2018 Apr 25;56(5). pii: e00050-18. doi: 10.1128/JCM.00050-18. Print 2018 May. PubMed PMID: 29444829; PubMed Central PMCID: PMC5925695.
 
Palacios-Baena ZR, Gutiérrez-Gutiérrez B, Calbo E, Almirante B, Viale P, Oliver A, Pintado V, Gasch O, Martínez-Martínez L, Pitout J, Akova M, Peña C, Molina Gil-Bermejo J, Hernández A, Venditti M, Prim N, Bou G, Tacconelli E, Tumbarello M, Hamprecht A, Giamarellou H, Almela M, Pérez F, Schwaber MJ, Bermejo J, Lowman W, Hsueh PR, Paño-Pardo JR, Torre-Cisneros J, Souli M, Bonomo RA, Carmeli Y, Paterson DL, Pascual Á, Rodríguez-Baño J; Spanish Network for Research in Infectious Diseases (REIPI)/European Study Group of Bloodstream Infections and Sepsis (ESGBIS)/INCREMENT Group. Empiric Therapy With Carbapenem-Sparing Regimens for Bloodstream Infections due to Extended-Spectrum β-Lactamase-Producing Enterobacteriaceae: Results From the INCREMENT Cohort.
Clin Infect Dis. 2017 Oct  30;65(10):1615-1623. doi: 10.1093/cid/cix606. PubMed PMID: 29020250; PubMed Central PMCID: PMC5849995.
 
Walther G, Stasch S, Kaerger K, Hamprecht A, Roth M, Cornely OA, Geerling G,Mackenzie CR, Kurzai O, von Lilienfeld-Toal M. Fusarium Keratitis in Germany.
J Clin Microbiol. 2017 Oct;55(10):2983-2995. doi: 10.1128/JCM.00649-17. PubMed PMID: 28747368; PubMed Central PMCID: PMC5625384.
 
Alanio A, Gits-Muselli M, Guigue N, Desnos-Ollivier M, Calderon EJ, Di Cave D, Dupont D, Hamprecht A, Hauser PM, Helweg-Larsen J, Kicia M, Lagrou K, Lengerova M, Matos O, Melchers WJG, Morio F, Nevez G, Totet A, White LP, Bretagne S.
Diversity of Pneumocystis jirovecii Across Europe: A Multicentre Observational Study.
EBioMedicine. 2017 Aug;22:155-163. doi: 10.1016/j.ebiom.2017.06.027
 
Hamprecht A, Morio F, Bader O, Le Pape P, Steinmann J, Dannaoui E.
Azole Resistance in Aspergillus fumigatus in Patients with Cystic Fibrosis: A Matter of Concern?
Mycopathologia. 2017 Jun 26. doi: 10.1007/s11046-017-0162-4.
 
Koroska F, Göttig S, Kaase M, Steinmann J, Gatermann S, Sommer J, Wille T, Plum G, Hamprecht A.
Comparison of Phenotypic Tests and an Immunochromatographic  Assay and Development of a New Algorithm for Detection of OXA-48-like Carbapenemases.
J Clin Microbiol. 2017 Mar;55(3):877-883. doi: 10.1128/JCM.01929-16.
 
Gruber TM, Göttig S, Mark L, Christ S, Kempf VA, Wichelhaus TA and Hamprecht A
Pathogenicity of pan-drug-resistant Serratia marcescens harbouring blaNDM-1.
J Antimicrob Chemother. 2014 Dec 2
 
Steinmann J, Hamprecht A, Vehreschild MJ, Cornely OA, Buchheidt D, Spiess B, Koldehoff M, Buer J, Meis JF, Rath PM.
Emergence of azole-resistant invasive aspergillosis in HSCT recipients in Germany.
J Antimicrob Chemother. 2015 May;70(5):1522-6. doi: 10.1093/jac/dku566.
 
Maschmeyer G, Carratalà J, Buchheidt D, Hamprecht A, Heussel CP, Kahl C, Lorenz J, Neumann S, Rieger C, Ruhnke M, Salwender H, Schmidt-Hieber M, Azoulay E; Infectious Diseases Working Party of the German Society of Hematology and Medical Oncology.
Diagnosis and antimicrobial therapy of lung infiltrates in febrile neutropenic patients (allogeneic SCT excluded): updated guidelines of the Infectious Diseases Working Party (AGIHO) of the German Society of Hematology and Medical Oncology (DGHO). Ann Oncol. 2015 Jan;26(1):21-33. doi: 10.1093/annonc/mdu192.
 
Fischer J, van Koningsbruggen-Rietschel S, Rietschel E, Vehreschild MJ, Wisplinghoff H, Krönke M, Hamprecht A.
Prevalence and molecular characterization of azole resistance in Aspergillus spp. isolates from German cystic fibrosis patients.
J Antimicrob Chemother. 2014 Jun;69(6):1533-6. doi: 10.1093/jac/dku009.
 
Hamprecht A, Christ S, Oestreicher T, Plum G, Kempf VA, Göttig S.
Performance of two MALDI-TOF MS systems for the identification of yeasts isolated from bloodstream infections and cerebrospinal fluids using a time-saving direct transfer protocol.
Med Microbiol Immunol. 2014 Apr;203(2):93-9. doi: 10.1007/s00430-013-0319-9.
 
Göttig S, Hamprecht AG, Christ S, Kempf VA, Wichelhaus TA.
Detection of NDM-7 in Germany, a new variant of the New Delhi metallo-β-lactamase with increased carbapenemase activity.
J Antimicrob Chemother. 2013 Aug;68(8):1737-40. doi: 10.1093/jac/dkt088
 
Hamprecht A, Poirel L, Göttig S, Seifert H, Kaase M, Nordmann P.
Detection of the carbapenemase GIM-1 in Enterobacter cloacae in Germany.
J Antimicrob Chemother. 2013 Mar;68(3):558-61. doi: 10.1093/jac/dks447
 
Hamprecht A, Buchheidt D, Vehreschild JJ, Cornely OA, Spiess B, Plum G, Halbsguth TV, Kutsch N, Stippel D, Kahl P, Persigehl T, Steinbach A, Bos B, Hallek M, Vehreschild MJ.
Azole-resistant invasive aspergillosis in a patient with acute myeloid leukaemia in Germany. Euro Surveill. 2012 Sep 6;17(36):20262.