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AG Hamprecht
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AG Hamprecht

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Group Leader: Univ. Prof. Dr. med. Axel Hamprecht

Professur für Antibiotika-Resistenzen von Gram-negativen Pathogenen
Phone: +49 221 478 32162
E-Mail: [Email protection active, please enable JavaScript.]

Co-workers:
Christopher Greissl
Ahmad Saleh
Yvonne Stelzer
Dr. Philipp Thelen
Dr. rer. nat. Thorsten Wille

MD students
Hanna Gistl
Maria Meier
Tsvetan Tsvetkov


Research Topic:
Resistance mechanisms in gram-negative bacteria

Detection and resistance of medically important fungi

Resistance mechanisms in gram-negative bacteria
Resistance to antibiotics has dramatically increased in recent years, especially among gram-negative bacteria (e.g., Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa). These bacteria cause life threatening infections (e.g. sepsis, pneumonia) and can be transmitted from patient to patient.

In the last years, we witness an increasing resistance to commonly used beta-lactam antibiotics, especially to extended spectrum cephalosporins. Resistance is commonly caused by extended spectrum beta lactamases that can hydrolyze many beta lactams.

Another threat which is still rare in Germany is the resistance to carbapenems (e.g., ertapenem, meropenem, imipenem), which is caused by carbapenemases such as NDM (New Delhi metallo-beta-lactamase), KPC, VIM, OXA-48 and others.

The main interest of our group is to identify and characterize resistant Gram-negative bacteria and to understand why some species and clones can disseminate successfully.

Furthermore, we work on the rapid detection of carbapenemase-producing Enterobacteriaceae directly from clinical specimen.

azole-resistant-aspergillus-fumigatus.jpg

Azole-resistant Aspergillus fumigatus (TR46/Y121F/T289A mutation)

Detection and resistance of medically important fungi
With the growing number of immune-compromised patients, we see an increasing number of infections by opportunistic pathogens, especially fungi. Invasive fungal infections such as aspergillosis show a very high mortality and are particularly difficult to treat. This problem has been aggravated by an increasing resistance to triazole antifungals, the mainstay of aspergillosis therapy. This phenomenon has only been observed in the last years and is likely caused the use of azole fungicides in agriculture.

Our current research focuses on azole resistance in Aspergillus spp. and the detection and resistance of “rare” fungi, such as Fusarium spp.


Brief description in German
Unsere Arbeitsgruppe beschäftigt sich mit Resistenzen und Resistenzmechanismen von gram-negativen Bakterien und medizinisch wichtigen Pilzen.

Resistenzen gegenüber Antibiotika und Antimykotika haben in den letzten Jahren dramatisch zugenommen. Dies ist u.a. bedingt durch den zu häufigen Einsatz von Antibiotika (Klinik, ambulant, Tierhaltung) und von Antimykotika (Landwirtschaft).

Unsere Arbeitsgruppe hat sich zum Ziel gesetzt, resistente Stämme nachzuweisen und zu charakterisieren sowie die zugrundeliegenden Resistenzmechanismen aufzuklären. Unser Hauptinteresse liegt hierbei bei Antibiotikaresistenzen gegenüber Betalaktamen (z.B. durch ESBLs oder Carbapenemasen wie NDM, KPC, OXA-48 u.a.) und Antimykotikaresistenzen bei Aspergillus spp. gegenüber Triazolen.

Offene Stellen
Offene Stellen sind unter der Rubrik „Stellen“ auf der Homepage des IMMIH aufgeführt.
Für Projekte im Rahmen von Master- oder Diplomarbeiten suchen wir immer engagierte und hoch motivierte Studenten. Die Durchführung einer Bachelorarbeit ist prinzipiell möglich, jedoch ist ein Einsatz von mindestens 4 Monaten im Labor erforderlich.
Für diese und andere Anfragen wenden Sie sich bitte direkt an den Gruppenleiter.

 

Selected publications

M, Behre G, Buchheidt D, Christopeit M, Hamprecht A, Heinz W, Heussel CP, Horger M, Kurzai O, Karthaus M, Löffler J, Maschmeyer G, Penack O, Rieger C, Rickerts V, Ritter J, Schmidt-Hieber M, Schuelper N, Schwartz S, Ullmann A, Vehreschild JJ, von Lilienfeld-Toal M, Weber T, Wolf HH.
Diagnosis of Invasive Fungal Diseases in Haematology and Oncology. 2018 Update of the Recommendations of the Infectious Diseases Working Party of the German Society for Hematology and Medical Oncology (AGIHO).
Mycoses. 2018 Aug 11. doi: 10.1111/myc.12838. [Epub ahead of print] Review. PubMed PMID: 30098069.

Pietsch M, Irrgang A, Roschanski N, Brenner Michael G, Hamprecht A, Rieber H, Käsbohrer A, Schwarz S, Rösler U, Kreienbrock L, Pfeifer Y, Fuchs S, Werner G; RESET Study Group.
Whole genome analyses of CMY-2-producing Escherichia coli isolates from humans, animals and food in Germany.
BMC Genomics. 2018 Aug 9;19(1):601. doi: 10.1186/s12864-018-4976-3. PubMed PMID: 30092762

Russo A, Falcone M, Gutiérrez-Gutiérrez B, Calbo E, Almirante B, Viale PL, Oliver A, Ruiz-Garbajosa P, Gasch O, Gozalo M, Pitout J, Akova M, Peña C, Cisneros JM, Hernández-Torres A, Farcomeni A, Prim N, Origüen J, Bou G, Tacconelli E, Tumbarello M, Hamprecht A, Karaiskos I, de la Calle C, Pérez F, Schwaber MJ, Bermejo J, Lowman W, Hsueh PR, Mora-Rillo M, Rodriguez-Gomez J, Souli M, Bonomo RA, Paterson DL, Carmeli Y, Pascual A, Rodríguez-Baño J, Venditti M; REIPI/ESGBIS/INCREMENT investigators.
Predictors of outcome in patients with severe sepsis or septic shock due to extended-spectrum β-lactamase-producing Enterobacteriaceae.
Int J Antimicrob Agents. 2018 Jun 30. pii: S0924-8579(18)30186-9. doi: 10.1016/j.ijantimicag.2018.06.018. [Epub ahead of print] PubMed PMID: 29969692.

Seufert R, Sedlacek L, Kahl B, Hogardt M, Hamprecht A, Haase G, Gunzer F, Haas A, Grauling-Halama S, MacKenzie CR, Essig A, Stehling F, Sutharsan S, Dittmer S, Killengray D, Schmidt D, Eskandarian N, Steinmann E, Buer J, Hagen F, Meis JF,Rath PM, Steinmann J.
Prevalence and characterization of azole-resistant Aspergillus fumigatus in patients with cystic fibrosis: a prospective multicenter study in Germany.
J Antimicrob Chemother. 2018 Aug 1;73(8):2047-2053. doi: 10.1093/jac/dky147. PubMed PMID: 29684150.

Thelen P, Hornberg K, Hamprecht A.
Efficient processing of MRSA screening specimens by a modified inoculation protocol.
Eur J Clin Microbiol Infect Dis. 2018 Jul 6. doi: 10.1007/s10096-018-3319-2. PubMed PMID: 29980897.

Hamprecht AG, Göttig S.
Häufigkeit und Vorkommen multiresistenter gramnegativer Erreger: 3MRGN/4MRGN.
Dtsch Med Wochenschr. 2018 May;143(9):625-633. doi: 10.1055/s-0043-113597. PubMed PMID: 29684929.

Saleh A, Göttig S, Hamprecht AG.
Multiplex Immunochromatographic Detection of OXA-48, KPC, and NDM Carbapenemases: Impact of Inoculum, Antibiotics, and Agar.
J Clin Microbiol. 2018 Apr 25;56(5). pii: e00050-18. doi: 10.1128/JCM.00050-18. Print 2018 May. PubMed PMID: 29444829; PubMed Central PMCID: PMC5925695.

Palacios-Baena ZR, Gutiérrez-Gutiérrez B, Calbo E, Almirante B, Viale P, Oliver A, Pintado V, Gasch O, Martínez-Martínez L, Pitout J, Akova M, Peña C, Molina Gil-Bermejo J, Hernández A, Venditti M, Prim N, Bou G, Tacconelli E, Tumbarello M, Hamprecht A, Giamarellou H, Almela M, Pérez F, Schwaber MJ, Bermejo J, Lowman W, Hsueh PR, Paño-Pardo JR, Torre-Cisneros J, Souli M, Bonomo RA, Carmeli Y, Paterson DL, Pascual Á, Rodríguez-Baño J; Spanish Network for Research in Infectious Diseases (REIPI)/European Study Group of Bloodstream Infections and Sepsis (ESGBIS)/INCREMENT Group. Empiric Therapy With Carbapenem-Sparing Regimens for Bloodstream Infections due to Extended-Spectrum β-Lactamase-Producing Enterobacteriaceae: Results From the INCREMENT Cohort.
Clin Infect Dis. 2017 Oct  30;65(10):1615-1623. doi: 10.1093/cid/cix606. PubMed PMID: 29020250; PubMed Central PMCID: PMC5849995.

Walther G, Stasch S, Kaerger K, Hamprecht A, Roth M, Cornely OA, Geerling G,Mackenzie CR, Kurzai O, von Lilienfeld-Toal M. Fusarium Keratitis in Germany.
J Clin Microbiol. 2017 Oct;55(10):2983-2995. doi: 10.1128/JCM.00649-17. PubMed PMID: 28747368; PubMed Central PMCID: PMC5625384.

Jazmati, N., Jazmati T, Hamprecht A.
Importance of pre-enrichment for detection of third-generation cephalosporin-resistant Enterobacteriaceae (3GCREB) from rectal swabs
Eur J Clin Microbiol Infect Dis 2017, May 5

Alanio A, Gits-Muselli M, Guigue N, Desnos-Ollivier M, Calderon EJ, Di Cave D, Dupont D, Hamprecht A, Hauser PM, Helweg-Larsen J, Kicia M, Lagrou K, Lengerova M, Matos O, Melchers WJG, Morio F, Nevez G, Totet A, White LP, Bretagne S.
Diversity of Pneumocystis jirovecii Across Europe: A Multicentre Observational Study.
EBioMedicine. 2017 Aug;22:155-163. doi: 10.1016/j.ebiom.2017.06.027

Hamprecht A, Morio F, Bader O, Le Pape P, Steinmann J, Dannaoui E.
Azole Resistance in Aspergillus fumigatus in Patients with Cystic Fibrosis: A Matter of Concern?
Mycopathologia. 2017 Jun 26. doi: 10.1007/s11046-017-0162-4.

Weber M, Schaer J, Walther G, Kaerger K, Steinmann J, Rath PM, Spiess B, Buchheidt D, Hamprecht A, Kurzai O. FunResDB-A web resource for genotypic susceptibility testing of Aspergillus fumigatus.
Med Mycol. 2017 Mar 11. doi: 10.1093/mmy/myx015.

Koroska F, Göttig S, Kaase M, Steinmann J, Gatermann S, Sommer J, Wille T, Plum G, Hamprecht A.
Comparison of Phenotypic Tests and an Immunochromatographic  Assay and Development of a New Algorithm for Detection of OXA-48-like Carbapenemases.
J Clin Microbiol. 2017 Mar;55(3):877-883. doi: 10.1128/JCM.01929-16.

Koehler P, Hamprecht A, Bader O, Bekeredjian-Ding I, Buchheidt D, Doelken G, Elias J, Haase G, Hahn-Ast C, Karthaus M, Kekulé A, Keller P, Kiehl M, Krause SW, Krämer C, Neumann S, Rohde H, La Rosée P, Ruhnke M, Schafhausen P, Schalk E, Schulz K, Schwartz S, Silling G, Staib P, Ullmann A, Vergoulidou M, Weber T, Cornely OA, Vehreschild MJ.
Epidemiology of invasive aspergillosis and azole resistance in patients with acute leukaemia: the SEPIA Study.
Int J Antimicrob Agents. 2017 Feb;49(2):218-223. doi: 10.1016/j.ijantimicag.2016.10.019.

Gruber TM, Göttig S, Mark L, Christ S, Kempf VA, Wichelhaus TA and Hamprecht A
Pathogenicity of pan-drug-resistant Serratia marcescens harbouring blaNDM-1.
J Antimicrob Chemother. 2014 Dec 2

Hamprecht A and Göttig S
Treatment of Infections Caused by Carbapenem-Resistant Enterobacteriaceae
Curr Treat Options Infect Dis Aug 2014, DOI 10.1007/s40506-014-0029-x

Steinmann J, Hamprecht A, Vehreschild MJ, Cornely OA, Buchheidt D, Spiess B, Koldehoff M, Buer J, Meis JF, Rath PM.
Emergence of azole-resistant invasive aspergillosis in HSCT recipients in Germany.
J Antimicrob Chemother. 2015 May;70(5):1522-6. doi: 10.1093/jac/dku566.

Vehreschild MJ, Hamprecht A, Peterson L, Schubert S, Häntschel M, Peter S, Schafhausen P, Rohde H, Lilienfeld-Toal MV, Bekeredjian-Ding I, Libam J, Hellmich M, Vehreschild JJ, Cornely OA, Seifert H.
A multicentre cohort study on colonization and infection with ESBL-producing Enterobacteriaceae in high-risk patients with haematological malignancies.
J Antimicrob Chemother. 2014 Dec;69(12):3387-92. doi: 10.1093/jac/dku305

Maschmeyer G, Carratalà J, Buchheidt D, Hamprecht A, Heussel CP, Kahl C, Lorenz J, Neumann S, Rieger C, Ruhnke M, Salwender H, Schmidt-Hieber M, Azoulay E; Infectious Diseases Working Party of the German Society of Hematology and Medical Oncology.
Diagnosis and antimicrobial therapy of lung infiltrates in febrile neutropenic patients (allogeneic SCT excluded): updated guidelines of the Infectious Diseases Working Party (AGIHO) of the German Society of Hematology and Medical Oncology (DGHO). Ann Oncol. 2015 Jan;26(1):21-33. doi: 10.1093/annonc/mdu192.

Fischer J, van Koningsbruggen-Rietschel S, Rietschel E, Vehreschild MJ, Wisplinghoff H, Krönke M, Hamprecht A.
Prevalence and molecular characterization of azole resistance in Aspergillus spp. isolates from German cystic fibrosis patients.
J Antimicrob Chemother. 2014 Jun;69(6):1533-6. doi: 10.1093/jac/dku009.

Hamprecht A, Christ S, Oestreicher T, Plum G, Kempf VA, Göttig S.
Performance of two MALDI-TOF MS systems for the identification of yeasts isolated from bloodstream infections and cerebrospinal fluids using a time-saving direct transfer protocol.
Med Microbiol Immunol. 2014 Apr;203(2):93-9. doi: 10.1007/s00430-013-0319-9.

Göttig S, Hamprecht AG, Christ S, Kempf VA, Wichelhaus TA.
Detection of NDM-7 in Germany, a new variant of the New Delhi metallo-β-lactamase with increased carbapenemase activity.
J Antimicrob Chemother. 2013 Aug;68(8):1737-40. doi: 10.1093/jac/dkt088

Hamprecht A, Poirel L, Göttig S, Seifert H, Kaase M, Nordmann P.
Detection of the carbapenemase GIM-1 in Enterobacter cloacae in Germany.
J Antimicrob Chemother. 2013 Mar;68(3):558-61. doi: 10.1093/jac/dks447

Hamprecht A, Buchheidt D, Vehreschild JJ, Cornely OA, Spiess B, Plum G, Halbsguth TV, Kutsch N, Stippel D, Kahl P, Persigehl T, Steinbach A, Bos B, Hallek M, Vehreschild MJ.
Azole-resistant invasive aspergillosis in a patient with acute myeloid leukaemia in Germany. Euro Surveill. 2012 Sep 6;17(36):20262.