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AG Papadopoulou
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AG Papadopoulou

Willkommen bei der AG Papadopoulou!
Nachwuchsgruppe der Medizinischen Fakultät am Klinikum der Universität zu Köln

Group Leader: Dr. Nikoletta Papadopolou
Phone: +49 221 478-32034 [Email protection active, please enable JavaScript.]

Selected recent publications

Co-workers: 
Birgit Blissenbach (-32220/32102 [Email protection active, please enable JavaScript.] )
Alzbeta Machova (-32218/32102 [Email protection active, please enable JavaScript.] )
Sarah Wilmschen (-32218 [Email protection active, please enable JavaScript.] )

Alumni:
Claudia Freitag
Oliver Hendrich
Anne Miller
Dr. Anna Schnitger
Lazaros Vasilikos


Brief description in German:
Unsere Forschung:

MicroRNAs (miRNAs) sind kleine nicht-kodierende RNAs, welche an messenger-RNAs binden und zu deren Abbau oder translationaler Inhibition führen. Wir untersuchen die Rolle von microRNAs und RNAi bei Wirt-Erreger- Interaktionen.

Die Charakterisierung von microRNA Expressionsprofilen in infizierten myeloischen Zellen zeigte eine Hochregulierung 14 microRNAs nach Infektion mit Listeria monocytogenes. Unsere Ergebnisse deuten daraufhin, dass microRNAs Teil der Immunantwort gegen Listerien sind und die Immunantwort von Makrophagen und Mastzellen nach bakteriellen Infektionen regulieren. Dies führt wahrscheinlich zur Beseitigung von Bakterien durch Zellen des adaptiven Immunsystems.

Unser Ziel ist die Aufklärung der Funktion von microRNAs und Ziel- messenger-RNAs im Zuge von Bakterieninfektionen in vitro und in vivo. Hierzu verwenden wir eine Kombination aus biochemischen, bioinformatischen, zell- und molekularbiologischen Ansätzen sowie Mausmodelle.

 

Research topic:
Our scientific goal is to understand the molecular mechanisms that regulate host-pathogen interactions at post-transcriptional levels.

Upon infection, pathogen associated molecular patterns are detected by pattern recognition receptors on innate immune cells, which initiate a wide spectrum of responses from phagocytosis to production of cytokines, essential for elicitation of adaptive immunity and clearance of bacteria.

Listeria monocytogenes is a Gram-positive facultative intracellular bacterium which has been used as paradigm for host-pathogen interactions. Macrophages, neutrophils and mast cells are key players of the early phase of innate immune response upon infection with L. monocytogenes. Regulation of the innate immune responses to infection is known to be controlled at transcriptional level. However, our knowledge on the post-transcriptional regulation of gene-expression in the function of innate immune cells is far from comprehensive.

MicroRNAs (miRNAs) are small non-coding RNAs that bind to mRNAs resulting in mRNA degradation or translational inhibition. We study the role of microRNAs (miRNAs) and RNAi pathway components in host-pathogen interactions. Characterisation of the miRNA expression profile in infected myeloid cells showed that 14 miRNAs were significantly up-regulated upon L. monocytogenes infection.

Our findings suggest that miRNAs are part of the early vacuolar-dependent anti-listerial response and support the potential role of miRNAs in regulating the innate immune responses of macrophages and mast cells upon bacterial infections, which will eventually lead to clearance of bacteria by the adaptive immune system.

Our aim is to elucidate the function of miRNA and target mRNAs during the course of bacteria infection in vitro and in vivo. A combination of biochemical, cell/molecular biology, mouse genetics and bioinformatics approaches are employed.

Selected recent publications (2005-date):
Anna K. D. Schnitger, Alzbeta Machova, Roman Ulrich Mueller, Ariadne Androulidaki, Bernhard Schermer, Manolis Pasparakis, Martin Krönke, Nikoletta Papadopoulou. (2011) Listeria monocytogenes Infection in Macrophages Induces Vacuolar-Dependent Host miRNA Response. PLoS ONE 6(11): e27435. doi:10.1371/journal.pone.0027435

Huang M, Kempuraj D, Papadopoulou N, Kourelis T, Donelan J, Manola A, Theoharides TC. (2009) Urocortin induces interleukin-6 release from rat cardiomyocytes through p38 MAP kinase, ERK and NF-kappaB activation. J Mol Endocrinol 42(5):397-405.

M.A. Ermolaeva, M.C. Michallet, N. Papadopoulou, O. Utermoehlen, K. Kranidioti, G. Kollias, J. Tschopp, M. Pasparakis. (2008) Function of TRADD in tumor necrosis factor receptor 1 signaling and in TRIF-dependent inflammatory responses. Nat Immunol 9(9):1037-46.

Ansel KM, Rath N*, Pastor WA*, Lapan AD*, Glasmacher E, Wolf C, Smith L, Papadopoulou N, Lampetti ED, Tahiliani M, Ellwart JW, Shi Y, Kremmer E, Rao A, Heissmeyer V. (2008) Eri-1 catalyzes end processing of the 5.8S ribosomal RNA. Nature Structural and Molecular Biology 15(5):523-30 (*equal contribution)

Wegener E, Oeckinghaus A, Papadopoulou N, Lavitas L, Schmidt-Supprian M, Ferch U, Mak TW, Ruland J, Heissmeyer V, Krappmann D. (2006) Essential role for IkappaB kinase beta in remodeling Carma1-Bcl10-Malt1 complexes upon T cell activation. Molecular Cell 7;23(1):13-23.

Donelan J, Boucher W, Papadopoulou N, Lytinas M, Papaliodis D, Dobner P, Theoharides TC. (2006) Corticotropin-releasing hormone induces skin vascular permeability through a neurotensin-dependent process. Proc Natl Acad Sci U S A 103(20):7759-64.

N.G. Papadopoulou, L. Oleson, D. Kempuraj, J. Donelan, C.L. Cetrulo and T. C. Theoharides. (2005) Regulation of corticotropin-releasing hormone receptor- 2 expression in human cord blood-derived cultured mast cells. J Mol Endocrinol 35(3):R1-8.

N. Papadopoulou, D. Kalogeromitros, N. G. Staurianeas, D. Tiblalexi, T. C. Theoharides. (2005) Corticotropin-Releasing Hormone Receptor-1 and Histidine Decarboxylase Expression in Chronic Urticaria. J Invest Dermatol 125(5):952-5.

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